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Related Experiment Video

Updated: Nov 10, 2025

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
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Radiomics Biomarkers Correlate with CD8 Expression and Predict Immune Signatures in Melanoma Patients.

Lauren G Aoude1, Bernadette Z Y Wong2,3, Vanessa F Bonazzi4

  • 1The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, Queensland, Australia. l.aoude@uq.edu.au.

Molecular Cancer Research : MCR
|April 3, 2021
PubMed
Summary

Computed tomography (CT) image features from PET/CT scans can predict survival in metastatic melanoma patients. These imaging biomarkers correlate with tumor immune status and BRAF mutation, offering prognostic value.

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Area of Science:

  • Oncology
  • Radiology
  • Immunology

Background:

  • Metastatic melanoma treatment involves targeted therapy and immunotherapy, but complete response rates are limited.
  • Predictive and prognostic biomarkers are crucial for advanced melanoma, especially given tumor accessibility challenges.
  • Non-invasive imaging techniques are needed for biomarker development in late-stage melanoma.

Purpose of the Study:

  • To evaluate computed tomography (CT) image parameters from PET/CT scans as prognostic biomarkers for metastatic melanoma.
  • To investigate the association of CT image biomarkers with patient survival, tumor genomics, and immune microenvironment characteristics.

Main Methods:

  • Analysis of PET/CT scans from 52 patients with stage III/IV melanoma.
  • Evaluation of CT image parameters, including standard deviation and mean of positive pixels (MPP).
  • Correlation with whole-exome sequencing (BRAF mutation status), RNA sequencing (immune signatures), and multiplex immunofluorescence (CD8 expression).

Main Results:

  • High standard deviation and high mean of positive pixels (MPP) were associated with worse progression-free survival and overall survival.
  • High MPP correlated with BRAF mutation status.
  • CT biomarker MPP4 was linked to 'cold' immune signatures and worse survival; CD8 expression correlated with image biomarkers.

Conclusions:

  • CT image parameters derived from PET/CT scans show potential as cost-effective prognostic biomarkers for metastatic melanoma survival.
  • These CT biomarkers reflect the tumor immune microenvironment and genomic status, aiding in patient stratification and treatment strategy.