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LOSS OF CAS3 AND INCREASE OF BAX EXPRESSION ASSOCIATED WITH PROGRESSION OF CERVICAL INTRAEPITHELIAL NEOPLASIA.
G Pkhakadze1, Z Bokhua1, T Asatiani1
1Tbilisi State Medical University, Georgia.
Georgian Medical News
|April 5, 2021
Summary
Apoptosis markers Cas3 and Bax, along with proliferation marker Ki67, are significantly altered during cervical intraepithelial neoplasia (CIN) progression. These changes indicate potential biomarkers for monitoring cervical cancer development.
Area of Science:
- Oncology
- Cell Biology
Background:
- Apoptosis is crucial in human cancer development, including cervical carcinoma.
- Cervical intraepithelial neoplasia (CIN) represents a spectrum of pre-cancerous changes in the cervix.
Purpose of the Study:
- To analyze the expression of Caspase-3 (Cas3) and Bax proteins.
- To correlate Cas3 and Bax expression with proliferation index (Ki67) and Estrogen Receptor (ER) status.
- To investigate these markers during the progression of cervical intraepithelial neoplasia (CIN).
Main Methods:
- Studied 140 cervical specimens, categorized by co-infection status.
- Employed standard immunohistochemistry to detect Ki67, Cas3, Bax, and ER.
- Analyzed protein expression correlations with CIN progression.
Main Results:
- Cas3 expression decreased, while Bax expression increased with CIN progression.
- Bax expression negatively correlated with Cas3 and ER expression.
- Bax expression positively correlated with Ki67 expression.
Conclusions:
- Deregulation of apoptosis (increased Bax, decreased Cas3) and increased proliferation (Ki67) are linked to CIN progression.
- These protein expressions may serve as valuable biomarkers for CIN progression and cervical cancer development.
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