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A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal
Brandon Gallian1,2, Masoumeh Saber Zaeimian1,2, Derrick Hau3
1Department of Electrical and Biomedical Engineering, University of Nevada, Reno, NV, United States.
Frontiers in Physics
|April 5, 2021
Summary
A novel time-gated immunoassay detects Burkholderia pseudomallei capsular polysaccharide (CPS) using long-lived nanocrystals. This method achieves high sensitivity for diagnosing melioidosis, even in human serum samples.
Area of Science:
- Biotechnology
- Nanotechnology
- Immunodiagnostics
Background:
- Melioidosis is a serious infectious disease caused by Burkholderia pseudomallei.
- Accurate and sensitive detection of Burkholderia pseudomallei is crucial for timely diagnosis and treatment.
- Current diagnostic methods may require improvement in sensitivity and specificity.
Purpose of the Study:
- To develop a time-gated immunoassay platform for detecting Burkholderia pseudomallei capsular polysaccharide (CPS).
- To utilize novel Mn:AgZnInS/ZnS nanocrystals as signal transducers for enhanced detection.
- To evaluate the platform's performance in detecting CPS in complex biological matrices like human serum.
Main Methods:
- Development of a time-gated immunoassay.
- Synthesis and application of low-energy excitable, long-lived fluorescence Mn:AgZnInS/ZnS nanocrystals as signal transducers.
- Detection of Burkholderia pseudomallei capsular polysaccharide (CPS).
Main Results:
- The developed time-gated immunoassay platform demonstrated effective detection of Burkholderia pseudomallei CPS.
- The assay achieved a limit of detection of approximately 23 pg/ml for CPS spiked in human serum.
- The use of Mn:AgZnInS/ZnS nanocrystals provided a sensitive signal transduction mechanism.
Conclusions:
- A sensitive and specific time-gated immunoassay for Burkholderia pseudomallei CPS detection has been successfully developed.
- The platform shows promise for the rapid and accurate diagnosis of melioidosis.
- This approach offers a valuable tool for infectious disease diagnostics, particularly for bacterial antigens.
