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Updated: Nov 10, 2025

Evidence-based Knowledge Synthesis and Hypothesis Validation: Navigating Biomedical Knowledge Bases via Explainable AI and Agentic Systems
Published on: June 13, 2025
Classifier uncertainty: evidence, potential impact, and probabilistic treatment
Niklas Tötsch1, Daniel Hoffmann1
1Faculty of Biology, University of Duisburg-Essen, Essen, Germany.
Abstract:
Classifiers are often tested on relatively small data sets, which should lead to uncertain performance metrics. Nevertheless, these metrics are usually taken at face value. We present an approach to quantify the uncertainty of classification performance metrics, based on a probability model of the confusion matrix. Application of our approach to classifiers from the scientific literature and a classification competition shows that uncertainties can be surprisingly large and limit performance evaluation. In fact, some published classifiers may be misleading. The application of our approach is simple and requires only the confusion matrix. It is agnostic of the underlying classifier. Our method can also be used for the estimation of sample sizes that achieve a desired precision of a performance metric.
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