Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Current Status of Korean Medicine Treatment for Schizophrenia Spectrum Disorders: a nationwide survey of Korean medicine doctors.

Journal of pharmacopuncture·2026
Same author

Real-World patterns of Korean medicine and combined Korean-Western medicine use in patients with chronic cough at Korean medicine institutions: a nationwide cohort study.

BMC complementary medicine and therapies·2026
Same author

Machine learning-based prediction of herbal medicine response in functional dyspepsia: protocol for a randomized, assessor-blinded, multicenter trial.

Frontiers in medicine·2026
Same author

Feasibility and performance evaluation of PPG on a Galaxy Watch in continuous central blood pressure monitoring.

European heart journal. Digital health·2026
Same author

Barriers and facilitators to enforcing E-cigarette regulations for online sales.

Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco·2026
Same author

Tobacco use trends in South Korea, 2013-2023: Persistent disparities and emerging challenges in a repeated cross-sectional study.

Tobacco induced diseases·2026

Related Experiment Video

Updated: Nov 10, 2025

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.2K

Sensitive label-free imaging of brain samples using FxClear-based tissue clearing technique.

Boram Lee1, Eunsoo Lee1,2, June Hoan Kim1

  • 1Department of Anatomy, Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, Seoul 02841, Republic of Korea.

Iscience
|April 5, 2021
PubMed
Summary

This study introduces a label-free optical clearing method for brain slices. It enhances refractive index differences between tissues, enabling clear imaging without staining.

Keywords:
Biology Experimental MethodsPhysiologyTechniques in Neuroscience

More Related Videos

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain
06:52

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain

Published on: January 26, 2024

2.5K
ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
07:46

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Published on: December 31, 2016

12.6K

Related Experiment Videos

Last Updated: Nov 10, 2025

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.2K
Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain
06:52

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain

Published on: January 26, 2024

2.5K
ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
07:46

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Published on: December 31, 2016

12.6K

Area of Science:

  • Neuroscience
  • Biomedical Optics
  • Histology

Background:

  • Optical clearing techniques are vital for volume imaging of biological tissues.
  • Achieving homogeneous staining in thick tissue samples for microscopy remains a significant challenge.

Purpose of the Study:

  • To develop a label-free imaging method for brain slices by manipulating optical properties.
  • To enhance regional heterogeneity and refractive index differences for improved contrast.

Main Methods:

  • Utilized FxClear, a delipidation method, to preserve lipids in white matter (WM).
  • Optimized embedding media to modulate contrast between lipid-rich and extracellular matrix-rich areas.
  • Tailored clearing conditions to enhance refractive index (RI) differences between gray and white matter.

Main Results:

  • Successfully enhanced regional heterogeneity of optical properties in brain slices.
  • Demonstrated improved contrast between gray and white matter, and for pathological features.
  • Showcased the potential for label-free imaging using conventional light microscopy and optical coherence tomography.

Conclusions:

  • The proposed protocol offers a simple, reliable, and flexible approach for label-free imaging.
  • This method is readily implementable in routine histology laboratories.
  • Enhancing RI differences through tailored tissue clearing is effective for label-free microscopy.