Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlation of NPM1 immunohistochemistry with mutation subtypes, clinicopathologic variables and molecular testing in AML with NPM1 mutation.

Human pathology·2026
Same author

Targeted molecular profiling uncovers true ceruminous adenomas with HMGA2::WIF1 and ceruminous syringocystadenoma papilliferum with BRAF V600E.

Virchows Archiv : an international journal of pathology·2025
Same author

High Glucose Contribution to the TCA Cycle Is a Feature of Aggressive Non-Small Cell Lung Cancer in Patients.

Cancer discovery·2025
Same author

Recurrent cytogenetic abnormalities reveal alterations that promote progression and transformation in myelodysplastic syndrome.

Cancer genetics·2024
Same author

Comparison of 2 T-Cell Receptor-γ Clonality Assays on Skin Biopsies Suspicious for Mycosis Fungoides.

The American Journal of dermatopathology·2024
Same author

Serum D-2-hydroxyglutarate and the ratio of D-2HG/L-2HG predict <i>IDH</i> mutation in acute myeloid leukemia.

EJHaem·2023

Related Experiment Video

Updated: Nov 10, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

5.0K

Digital Droplet PCR for SARS-CoV-2 Resolves Borderline Cases.

Jing Xu1, Timothy Kirtek1, Yan Xu1

  • 1Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

American Journal of Clinical Pathology
|April 6, 2021
PubMed
Summary

Droplet digital PCR (ddPCR) accurately detects SARS-CoV-2, confirming weak positive results. This sensitive method is effective for evaluating borderline positive or suspected false-negative severe acute respiratory syndrome coronavirus 2 cases.

Keywords:
COVID-19Digital droplet polymerase chain reactionReal-time polymerase chain reactionSARS-CoV-2

More Related Videos

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
08:26

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

1.9K
Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
04:43

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

Published on: October 11, 2024

2.4K

Related Experiment Videos

Last Updated: Nov 10, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

5.0K
Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
08:26

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

1.9K
Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
04:43

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

Published on: October 11, 2024

2.4K

Area of Science:

  • Molecular Diagnostics
  • Virology
  • Biotechnology

Background:

  • The Bio-Rad SARS-CoV-2 ddPCR Kit was the first FDA-approved droplet digital PCR assay but lacked clinical validation.
  • Droplet digital PCR (ddPCR) offers potential for enhanced sensitivity in detecting viral RNA.

Purpose of the Study:

  • To clinically validate the Bio-Rad Triplex Probe ddPCR Assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection.
  • To evaluate the ddPCR assay's performance in confirming weak-positive SARS-CoV-2 results.

Main Methods:

  • Clinical validation of the Bio-Rad Triplex Probe ddPCR Assay.
  • Determination of the limit of detection using serial dilutions of SARS-CoV-2 RNA.
  • Comparison of ddPCR results with an FDA-validated RT-PCR assay on 48 positive and 30 negative specimens, plus 10 borderline cases.

Main Results:

  • The limit of detection was 50 copies/mL (19/20 positive).
  • The ddPCR assay demonstrated 97.9% positive percent agreement (47/48) and 100% negative percent agreement (30/30).
  • Nine out of ten borderline positive cases were confirmed positive by ddPCR when tested in triplicate.

Conclusions:

  • Droplet digital PCR (ddPCR) is an accurate method for SARS-CoV-2 detection with superior sensitivity for viral RNA.
  • ddPCR can definitively evaluate borderline positive cases and suspected false-negative results.
  • Clinical validation supports the use of ddPCR for reliable SARS-CoV-2 diagnostics.