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Affinity labeling by a photoreactive GTP analogue of the delta-subunit of eukaryotic initiation factor eIF-2 in
U A Bommer1, M M Salimans, T V Kurzchalia
1Department of Cell Physiology, Academy of Sciences of the GDR, Berlin-Buch.
Biochemistry International
|March 1, 1988
Abstract:
The interaction of GTP with initiation factor eIF-2 in different complexes was studied by affinity labeling using a derivative of [3H]GTP carrying a photoreactive group in the alpha-phosphate moiety. In the binary complex [eIF-2.GTP analogue], in the ternary complex [eIF-2.GTP analogue.Met-tRNAf] as well as in the eIF-2. eIF-2B complex the alpha-subunit of eIF-2 was found to be specifically labeled. GTP is concluded to interact during polypeptide chain initiation with the alpha-subunit of eIF-2 at least by its alpha-phosphate group.