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Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device
Published on: December 27, 2024
Carboxymethyl chitosan hydrogel formulations enhance the healing process in experimental partial-thickness
Randys Caldeira Gonçalves1, Roberta Signini2, Luciana Martins Rosa3
1PhD. Universidade Federal de Goiás - Instituto de Patologia Tropical e Saúde Pública - Programa de Pós-Graduação em Medicina Tropical e Saúde Pública - Goiânia (GO), Brazil.
Purpose:
This study aimed to elaborate a hydrogel constituted by carboxymethyl chitosan (CMC), hyaluronic acid (HA) and silver (Ag) and to evaluate its healing effect on partial-thickness burn wounds experimentally induced in rats.
Methods:
CMC was obtained by chitosan reacting with monochloroacetic acid. The carboxymethylation was confirmed by Fourier-transform infrared spectroscopy and hydrogen nuclear magnetic resonance (NMR). Scanning electron microscopy was used to determine the morphologicalcharacteristics of chitosan and CMC. After the experimental burn wound induction, the animals (n = 126) were treated with different CMC formulations, had their occlusive dressings changed daily and were followed through 7, 14 and 30 days. Morphometric, macroscopic and microscopic aspects and collagen quantification were evaluated.
Results:
Significative wound contraction, granulation tissue formation, inflammatory infiltration and collagen fibers deposit throughout different phases of the healing process were observed in the CMC hydrogels treated groups.
Conclusions:
The results showed that, in the initial phase of the healing process, the most adequate product was the CMC/HA/Ag association, while in the other phases the CMC/HA association was the best one to promote the healing of burn wounds.
