Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 9, 2025

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
07:14

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Published on: October 21, 2021

4.3K

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics.

Helena García-Castro1, Nathan J Kenny1, Marta Iglesias2,3

  • 1Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.

Genome Biology
|April 8, 2021
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<b>Autolytinae (Annelida, Syllidae) from Australia</b>.

Zootaxa·2026
Same author

Neuronal microexons modulate arousal via the cAMP-PKA-CREB pathway in zebrafish.

Science advances·2026
Same author

Functional characterization of specialized immune cells in a cnidarian reveals an ancestral antiviral program.

Nature communications·2026
Same author

GeneExt: a gene model extension tool for enhanced single-cell RNA-seq analysis.

Bioinformatics (Oxford, England)·2026
Same author

A Field-Friendly, Non-Toxic Fixative for Integrated Morphological and Molecular Research in Non-Model Invertebrates.

Ecology and evolution·2026
Same author

ERGA-BGE reference genome of the lineid heteronemertean <i>Lineus lacteus</i> (Pilidiophora, Nemertea).

Open research Europe·2026

A new method called ACME (Acetic-Methanol) allows single-cell transcriptomics without dissociating live samples. This technique preserves RNA integrity, enables cryopreservation, and is accessible for broad use, accelerating cell type discovery.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Single-cell sequencing is vital for biological research.
  • Current methods require live cell dissociation, limiting sample handling and preservation.
  • There is a need for improved dissociation techniques that maintain sample integrity.

Purpose of the Study:

  • To introduce ACME (Acetic-Methanol), a novel dissociation method for single-cell transcriptomics.
  • To demonstrate that ACME simultaneously dissociates and fixes cells.
  • To evaluate the suitability of ACME-processed cells for downstream transcriptomic analysis.

Main Methods:

  • Developed ACME, a reagent-based dissociation and fixation protocol.
  • Assessed RNA integrity of ACME-treated cells.
Keywords:
Combinatorial indexingDissociationFixationPlanarianRNA-seqSPLiT-seqSingle-cell transcriptomics

More Related Videos

Mouse Wound Models and Preparation of Single-Cell Suspensions
04:03

Mouse Wound Models and Preparation of Single-Cell Suspensions

Published on: September 27, 2024

944
Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors
05:58

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

3.4K

Related Experiment Videos

Last Updated: Nov 9, 2025

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
07:14

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Published on: October 21, 2021

4.3K
Mouse Wound Models and Preparation of Single-Cell Suspensions
04:03

Mouse Wound Models and Preparation of Single-Cell Suspensions

Published on: September 27, 2024

944
Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors
05:58

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

3.4K
  • Tested cryopreservation, sorting, and permeability of ACME-processed cells.
  • Generated single-cell transcriptomic data using droplet-based and combinatorial barcoding methods.

    • Main Results:

    • ACME effectively dissociates and fixes cells for single-cell transcriptomics.
    • Cells processed with ACME exhibit high RNA integrity.
    • ACME-treated cells are suitable for multiple cryopreservation cycles, sorting, and permeabilization.
    • Successful transcriptomic data acquisition across different species and methods.

    Conclusions:

    • ACME is a versatile and accessible method for single-cell transcriptomics.
    • The technique overcomes limitations of live-cell dissociation, improving sample handling.
    • ACME will facilitate broader cell type characterization across diverse organisms.