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Updated: Nov 9, 2025

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ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics.

Helena García-Castro1, Nathan J Kenny1, Marta Iglesias2,3

  • 1Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.

Genome Biology
|April 8, 2021
PubMed
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This summary is machine-generated.

A new method called ACME (Acetic-Methanol) allows single-cell transcriptomics without dissociating live samples. This technique preserves RNA integrity, enables cryopreservation, and is accessible for broad use, accelerating cell type discovery.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Single-cell sequencing is vital for biological research.
  • Current methods require live cell dissociation, limiting sample handling and preservation.
  • There is a need for improved dissociation techniques that maintain sample integrity.

Purpose of the Study:

  • To introduce ACME (Acetic-Methanol), a novel dissociation method for single-cell transcriptomics.
  • To demonstrate that ACME simultaneously dissociates and fixes cells.
  • To evaluate the suitability of ACME-processed cells for downstream transcriptomic analysis.

Main Methods:

  • Developed ACME, a reagent-based dissociation and fixation protocol.
  • Assessed RNA integrity of ACME-treated cells.
Keywords:
Combinatorial indexingDissociationFixationPlanarianRNA-seqSPLiT-seqSingle-cell transcriptomics

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  • Tested cryopreservation, sorting, and permeability of ACME-processed cells.
  • Generated single-cell transcriptomic data using droplet-based and combinatorial barcoding methods.

    • Main Results:

    • ACME effectively dissociates and fixes cells for single-cell transcriptomics.
    • Cells processed with ACME exhibit high RNA integrity.
    • ACME-treated cells are suitable for multiple cryopreservation cycles, sorting, and permeabilization.
    • Successful transcriptomic data acquisition across different species and methods.

    Conclusions:

    • ACME is a versatile and accessible method for single-cell transcriptomics.
    • The technique overcomes limitations of live-cell dissociation, improving sample handling.
    • ACME will facilitate broader cell type characterization across diverse organisms.