Versatile Biosensing Toolkit Using an Electronic Particle Counter

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensing Technology

Background:

• Versatile biosensing toolkits are crucial for rapid and multiplexed detection of various analytes.
• Existing methods may lack sensitivity, broad detection range, or multiplexing capabilities.

Purpose of the Study:

• To develop a versatile biosensing toolkit using an electronic particle counter for sensitive, multiplexed detection.
• To demonstrate the toolkit's applicability across diverse targets and recognition strategies.

Main Methods:

• Implementation of an electrical resistance-based particle counter for analyte quantification.
• Utilizing antigen-antibody interactions, DNA hybridization, and polypeptide recognition for target assaying.
• Assaying small molecules, protein biomarkers, pathogen bacteria, and tumor cells.

Main Results:

• The biosensing toolkit achieved high sensitivity and a broad detection range (pg mL⁻¹ to μg mL⁻¹).
• Demonstrated multiplexed detection capabilities for simultaneous analysis of multiple targets.
• Verified versatility across small molecules, protein biomarkers, pathogen bacteria, and tumor cells.

Conclusions:

• The developed electronic particle counter-based biosensing toolkit is versatile, sensitive, and cost-effective.
• It enables rapid, multiplexed detection, showing significant potential for food safety testing and biomedical diagnosis.