Ex vivo interaction study of NaYF₄ :Yb,Er nanophosphors with isolated mitochondria

Ex vivo interaction of NaYF₄ :Yb,Er nanophosphors with isolated mitochondria has been investigated. The nanophosphors were synthesized using the hydrothermal method. The synthesized NaYF₄ :Yb,Er nanophosphors were characterized for physicochemical properties. The NaYF₄ :Yb,Er nanophosphors showed successful upconversion with excitation wavelength lying in the near-infrared region. The effect of synthesized NaYF₄ :Yb,Er nanophosphors on mitochondria isolated from the chicken heart tissue was examined through ROS generation capacity, membrane fluidity, and complex II activity. The exposer of NaYF₄ :Yb,Er nanophosphors to isolated mitochondria inhibits ROS generation activity as compared to control. The mitochondria membrane fluidity of the lipid bilayer and complex-II activity of mitochondria was observed to be unaltered after the interaction with NaYF₄ :Yb,Er nanoparticles. The results confirm that synthesized NaYF₄ :Yb,Er nanoparticles can be used as a safe contrast agent.