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Related Experiment Video

Updated: Nov 9, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

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Microfluidics and the quantification of biomolecular interactions.

Daniel E Otzen1, Alexander K Buell2, Henrik Jensen3

  • 1iNANO and Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 14, DK - 8000, Aarhus C, Denmark.

Current Opinion in Structural Biology
|April 8, 2021
PubMed
Summary
This summary is machine-generated.

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Microfluidic systems offer cost-effective in-solution analysis of species size and binding affinities. These methods, including Taylor dispersion analysis and microfluidic diffusional sizing, are valuable for studying biomolecular interactions.

Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Microfluidic systems offer advantages for in-solution analysis.
  • Laminar flow conditions enable precise measurements.
  • Modest sample costs are a key benefit.

Purpose of the Study:

  • To present microfluidic techniques for analyzing species size and binding affinities.
  • To highlight the utility of flow-induced dispersion analysis and microfluidic diffusional sizing.
  • To demonstrate their value as complements to existing biomolecular interaction analysis methods.

Main Methods:

  • Utilizing microfluidic systems under laminar flow.
  • Employing flow-induced dispersion analysis (Taylor dispersion analysis).
  • Implementing microfluidic diffusional sizing for phase transfer quantification.
Keywords:
Binding affinityFlow-induced dispersion analysisHydrodynamic radiusMicrofluidic diffusional sizingTaylor dispersion analysis

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Related Experiment Videos

Last Updated: Nov 9, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.2K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

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A Microfluidic Chip for ICPMS Sample Introduction
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Main Results:

  • Analysis of species sizes ranging from 0.5 to 1000 nm.
  • Resolution of different populations within samples.
  • Successful analysis in complex media with medium throughput.

Conclusions:

  • Microfluidic techniques provide valuable in-solution data on size and binding affinities.
  • Taylor dispersion analysis and microfluidic diffusional sizing are effective methods.
  • These approaches complement existing techniques for biomolecular interaction studies.