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Related Concept Videos

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Updated: Nov 9, 2025

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
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Clinically Compatible Fluorescence Microscopy Based on Moxifloxacin Antibiotic.

Seunghoon Lee1, Ki Hean Kim2,3

  • 1Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.

Advances in Experimental Medicine and Biology
|April 9, 2021
PubMed
Summary

Moxifloxacin, an antibiotic, can be used as a fluorescent cell-labeling agent for high-resolution tissue imaging. This novel application enhances cellular visualization in various tissues, aiding in medical diagnosis and surgical guidance.

Keywords:
Cell-labeling agentClinically compatibleFluorescence microscopyLinear and nonlinear fluorescence microscopyMoxifloxacinPreclinical studies

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Area of Science:

  • Biomedical Imaging
  • Microscopy
  • Pharmacology

Background:

  • Moxifloxacin is a clinically approved antibiotic with intrinsic fluorescence and good tissue penetration.
  • Traditional cell-labeling agents may have limitations in tissue penetration and imaging speed.
  • Exploring alternative applications of existing drugs can lead to innovative diagnostic tools.

Purpose of the Study:

  • To investigate the utility of moxifloxacin as a cell-labelling agent for high-resolution fluorescence tissue imaging.
  • To evaluate the performance of moxifloxacin-based imaging in various tissue types and microscopy techniques.
  • To assess the potential clinical compatibility and applications of this novel imaging approach.

Main Methods:

  • Utilized moxifloxacin as a fluorescent agent for cell labeling in tissue samples.
  • Employed both linear and nonlinear fluorescence microscopy techniques for imaging.
  • Tested imaging capabilities across diverse tissues including cornea, skin, intestines, and brain.

Main Results:

  • Achieved high-contrast cellular imaging in multiple tissue types using moxifloxacin.
  • Demonstrated enhanced contrast and high imaging speeds with moxifloxacin-based fluorescence microscopy.
  • Successfully applied the technique in preclinical studies for pathogen detection and tumor margin delineation.

Conclusions:

  • Moxifloxacin serves as an effective and intrinsically fluorescent cell-labelling agent for high-resolution tissue imaging.
  • This FDA-approved drug offers a clinically compatible platform for fluorescence microscopy, potentially aiding in diagnosis and surgical guidance.
  • Moxifloxacin-based imaging shows promise for applications in infectious diseases and oncology.