Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Nov 9, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Stem cell-based vascularization of microphysiological systems.

Shane Browne¹, Elisabeth L Gill¹, Paula Schultheiss¹

¹Department of Bioengineering and California Institute for Quantitative Biosciences (QB3), University of California at Berkeley, Berkeley, CA 94720, USA.

Stem Cell Reports

|April 9, 2021

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Drug Proarrhythmic Evaluation in a High Throughput Cardiac New Approach Methodology.

bioRxiv : the preprint server for biology·2026

Same author

Multi-hierarchical biofunctional polymeric biomaterial to promote wound closure.

Journal of materials science. Materials in medicine·2026

Same author

A novel chitosan-collagen bilayer scaffold prevents contraction and accelerates cutaneous repair in a rat splint-skin model.

Frontiers in bioengineering and biotechnology·2026

Same author

Dental, Oral and Craniofacial Tissue Regeneration Consortium (DOCTRC): An infrastructure for accelerating regenerative therapies from discovery to clinical impact.

Journal of clinical and translational science·2026

Same author

Scaffold-mediated miRNA-155 inhibition promotes regenerative macrophage polarisation leading to anti-inflammatory, angiogenic and neurogenic responses for wound healing.

Bioactive materials·2026

Same author

Repairing Volumetric Muscle Loss with Skeletal Muscle Units and Hyaluronic Acid Hydrogel in Rats.

Tissue engineering. Part A·2025

Same journal

Integration of the naive pluripotency gene network with the response to GSK3 inhibition by Tcf7l1-driven enhancer decommissioning.

Stem cell reports·2026

Same journal

Neural stem cells as potential mediators of prenatal dietary stress through epigenetic mechanisms.

Stem cell reports·2026

Same journal

BDNF regulates pituitary stem cell engagement toward precursor state.

Stem cell reports·2026

Same journal

Atg5 deficiency alters myofibroblast accumulation and alveolar regeneration in lung fibrosis.

Stem cell reports·2026

Same journal

aPKC-ζ III promotes trophoblast fusion by altering Par-3 interactions with Hippo signaling kinase LATS1.

Stem cell reports·2026

Same journal

A highly efficient method to differentiate CGRP-expressing peptidergic nociceptors from human induced pluripotent stem cells.

Stem cell reports·2026

See all related articles

Microphysiological systems (MPSs) use microfluidics and stem cells to model human physiology. This review highlights vascularizing MPSs with human induced pluripotent stem cell-derived cells for better disease modeling.

Area of Science:

Biotechnology
Regenerative Medicine
Tissue Engineering

Background:

Microphysiological systems (MPSs) are advanced models of human tissues and organs.
Human induced pluripotent stem cell (hiPSC) technology enables patient-specific disease modeling in MPSs.
Vasculature development and complexity are often simplified in current MPS designs.

Purpose of the Study:

To review the development of vasculature in MPSs.
To explore the use of hiPSC-derived vascular cells in MPSs.
To categorize and discuss current vascular MPS strategies for in vitro disease modeling.

Main Methods:

Review of native vasculature development and hiPSC-derived vascular cell generation.
Categorization of vascular MPS approaches: self-assembled, interface-focused, and 3D biofabricated.

More Related Videos

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Published on: December 13, 2024

Related Experiment Videos

Last Updated: Nov 9, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Published on: December 13, 2024

Discussion of the state-of-the-art in vascularized MPSs.

Main Results:

hiPSC-derived endothelial and perivascular cells offer significant potential for improving MPS vascularization.
Three strategic categories of vascular MPS designs have been identified.
Advancements in vascularizing MPSs enhance their physiological relevance for disease modeling.

Conclusions:

Vascularizing MPSs with hiPSC-derived cells is crucial for accurate in vitro disease modeling.
Further interdisciplinary research can unlock new opportunities in vascular MPS development.
Optimized vascular components will increase the predictive power of MPS for human health.