Related Experiment Video

Updated: Nov 9, 2025

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

8.3K

The Pseudomonas aeruginosa PAAR2 cluster encodes a putative VRR-NUC domain-containing effector

Shuangyue Wang1, Zhi Geng2, Heng Zhang2

  • 1Institutes of Physical Science and Information Technology, Anhui University, Hefei, China.

The FEBS Journal
|April 10, 2021
PubMed

Insights

Researchers identified a novel effector-immunity pair, TseV-TsiV, in Pseudomonas aeruginosa. This discovery sheds light on the bacterial type VI secretion system

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Structural Biology

Background:

  • The bacterial type VI secretion system (T6SS) is crucial for inter-bacterial competition and host infection, delivering toxic effectors.
  • Cognate immunity proteins protect bacteria from self-inflicted effector toxicity.
  • Pseudomonas aeruginosa possesses five proline-alanine-alanine-arginine (PAAR) proteins, with some facilitating effector delivery.

Purpose of the Study:

  • To investigate a putative novel effector, TseV, containing a virus-type replication-repair nuclease domain, encoded by the P. aeruginosa PAAR2 cluster.
  • To determine the crystal structure of the putative cognate immunity protein, TsiV.
  • To propose TseV-TsiV as a novel effector-immunity (E-I) pair and explore the functions of other PAAR2 cluster proteins.

Main Methods:

  • X-ray crystallography was used to determine the 1.6 Å resolution structure of TsiV.
  • Sequence and structural comparisons were employed to analyze the TseV-TsiV interaction.
  • Bioinformatic analysis of the PAAR2 cluster and its encoded proteins.

Main Results:

  • The crystal structure of the putative immunity protein TsiV was solved at 1.6 Å resolution.
  • Structural and sequence analyses suggest that TseV and TsiV form a novel effector-immunity pair.
  • The study highlights the under-investigated PAAR2 cluster in P. aeruginosa.

Conclusions:

  • The TseV-TsiV system represents a newly identified effector-immunity pair within the P. aeruginosa T6SS.
  • This finding expands our understanding of T6SS diversity and function.
  • Further research into the PAAR2 cluster may reveal additional mechanisms of bacterial virulence and competition.
Keywords:
VRR-NUC domainX-ray crystallographyeffector-immunity pairproline-alanine-alanine-arginine repeatstype VI secretion system

More Related Videos

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection08:34

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Published on: January 8, 2020

7.0K
Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis08:57

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Published on: March 10, 2014

12.6K

Related Concept Videos

The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
364
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
194
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
325
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.0K
Bacterial Phylum Verrucomicrobiota01:26

Bacterial Phylum Verrucomicrobiota

The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
258
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
519