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Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos
Published on: August 25, 2019
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Evaluation of 3,4,4,9-trichlorocarbanilide to zebrafish developmental toxicity based on transcriptomics analysis
Liting He1, Yuanyao Chen1, Zhiyong Hu1
1Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei, 430030, PR China.
Chemosphere
|April 10, 2021
Summary
Triclocarban (TCC) exposure causes significant cardiac developmental toxicity in zebrafish embryos, leading to heart abnormalities and decreased heart rates. This toxicity is linked to aryl hydrocarbon receptor (AhR) activation and altered gene expression.
Area of Science:
- Environmental Toxicology
- Developmental Biology
- Zebrafish Embryology
Background:
- Triclocarban (TCC) is an endocrine-disrupting, persistent, and bioaccumulating organic pollutant.
- Limited studies exist on TCC's toxicological mechanisms, particularly concerning embryo development.
Purpose of the Study:
- To investigate the cardiac developmental toxicity of Triclocarban (TCC) in zebrafish embryos.
- To explore the toxicological mechanisms underlying TCC-induced cardiac defects.
Main Methods:
- Zebrafish embryos were exposed to varying concentrations of TCC.
- Liquid chromatography-tandem mass spectrometry quantified embryonic TCC levels.
- Transcriptome sequencing identified differential gene expression patterns.
Main Results:
- Exposure to 300 μg/L TCC resulted in decreased heart rates, increased heart abnormalities, and pericardial effusion in zebrafish embryos.
- Transcriptome analysis revealed significant upregulation of aryl hydrocarbon receptor (AhR) activation and cyp-related genes.
- These changes were associated with impaired embryonic heart development, tissue edema, and hemorrhage.
Conclusions:
- Triclocarban (TCC) exhibits significant cardiac teratogenic and developmental toxicity in zebrafish embryos.
- AhR activation is implicated in the observed cardiac toxicity.
- Transcriptome data aids in assessing TCC exposure risks, warranting further investigation into TCC-AhR interactions.
