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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Capture-SELEX for aptamer selection: A short review.

Chen Lyu1, Imran Mahmood Khan2, Zhouping Wang2

  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China.

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Capture-SELEX is an advanced aptamer selection method ideal for small molecules. This review highlights recent progress, applications, and future potential of this powerful screening technique for aptasensor development.

Keywords:
AptamerCapture-SELEXLibrary immobilization

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Aptamers, selected via Systematic Evolution of Ligands by Exponential Enrichment (SELEX), are crucial tools in basic research.
  • Traditional SELEX often faces challenges, particularly when selecting aptamers against small molecules.
  • Capture-SELEX offers a novel approach by immobilizing oligonucleotides instead of target molecules.

Purpose of the Study:

  • To provide an updated review of recent advancements in Capture-SELEX technology.
  • To emphasize key aspects of the Capture-SELEX protocol and its diverse applications.
  • To discuss the limitations and future prospects of Capture-SELEX for aptasensor discovery.

Main Methods:

  • Review of literature focusing on Capture-SELEX methodology.
  • Analysis of Capture-SELEX protocol variations and optimization strategies.
  • Examination of case studies demonstrating Capture-SELEX applications for small molecule aptamer isolation.

Main Results:

  • Capture-SELEX facilitates the isolation of high-affinity aptamers against small molecules, overcoming limitations of conventional SELEX.
  • Recent advances have refined Capture-SELEX protocols, enhancing efficiency and success rates.
  • The method shows significant promise for developing novel aptasensors for various applications.

Conclusions:

  • Capture-SELEX represents a significant advancement in aptamer selection, particularly for challenging targets like small molecules.
  • Further research and optimization of Capture-SELEX protocols will accelerate aptasensor development.
  • This review aims to inspire researchers to adopt and improve Capture-SELEX for future discoveries.