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Updated: Nov 9, 2025
Roux-en-Y Gastric Bypass Operation in Rats
Published on: June 11, 2012
Bariatric surgery induces morphological changes in the extensor digitorum longus muscle in the offspring of obese
C Kuhn1, C Covatti1, L F C Ribeiro1
1Master's Program in Biosciences and Health, State University of Western Paraná (UNIOESTE), Cascavel, PR, Brazil.
Introduction:
The effects of the maternal nutritional environment on the growth and metabolism of the offspring, and its impacts on health in adult life are defined as metabolic programming. Thus, the objective of this study was to evaluate the effects of Roux-en-Y gastric bypass (RYGB) on the morphology of muscle fiber and neuromuscular junction (NMJ) of the offspring of rats submitted to RYGB.
Methods:
Three-week-old Wistar rats were separated into two groups: 1) CAF SHAM which received a cafeteria diet and was submitted to a sham operation and 2) CAF RYGB, which received a cafeteria diet and was submitted to RYGB. The first generation (F1) offspring (male) was named according to the treatment of mothers as CAF SHAM-F1 and CAF RYGB-F1 and received a standard diet after weaning. At 17 weeks, the animals were euthanized, and the extensor digitorum longus muscle (EDL) was collected and processed in light microscopy and transmission electron microscopy for morphological and morphometric analysis.
Results:
The CAF RYGB-F1 group showed a reduction in the weight of the EDL muscle and also a reduction in the area of type I, IIa and IIb fibers and a nucleus/fiber ratio. This same group also showed an increase in the capillary density and myofibrillar disorganization and in the Z-line, as well as a reduction in the area of the NMJs.
Conclusion:
The RYGB surgery in mothers produced morphological changes in the skeletal striated muscles of the offspring.
