Luteal phase sera and progesterone enhance sperm penetration in the hamster egg assay

The hormonal factors responsible for variation in sperm penetration rates of zona-free hamster ova caused by the use of human sera were investigated. Sera obtained at different times in the menstrual cycle, 17-beta estradiol (E2), or progesterone (P) was added to Biggers Whitten and Whittingham (BWW) medium used for the sperm penetration assay. Luteal phase sera enhanced egg penetration significantly when compared with either follicular or preovulatory sera (48.8% versus 23% and 25%; P less than 0.01). E2 at concentrations of 2 pg/ml to 2 X 10(6) pg/ml had no effect on sperm motility or egg penetration. Addition of P to control media at final concentrations of 0.5 to 50 ng/ml caused a significant elevation of egg penetration (68% to 91% to 96%; P less than 0.01) without affecting sperm motility. Since P has been shown to have a direct effect on plasma membranes and to bind to spermatozoan plasma membrane, the authors propose that P could have a physiologic role during capacitation or sperm egg interaction in vivo.