Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.1K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.1K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.4K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversible Addition-Fragmentation Chain-Transfer Aqueous Emulsion Polymerization Observed by Transmission Electron Microscopy.

Journal of the American Chemical Society·2026
Same author

Martini 3 Coarse-Grain Model For Linear Perfluoroalkyl Substances.

Journal of chemical theory and computation·2026
Same author

Prediction of rheological properties via structure elucidation of solvated hydrogels.

Nature materials·2026
Same author

Rediscovering lost colors: film color restoration by hyperspectral imaging and cluster-based spectral correction algorithm (CBSCA).

RSC advances·2026
Same author

Heterobifunctional proteomimetic polymers for targeted degradation of MYC and KRAS.

Nature communications·2026
Same author

Exploring allomelanin: A comparative analysis via natural product extraction and synthesis.

Science advances·2026
Same journal

Methanol Partial Oxidation on Cu(111) and PtCu(111) Single-Atom Alloy Surfaces: Effect of Surface Oxygen Coverage on Selectivity.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Yb<sup>3+</sup>-Doped GaN Nanoceramics as a New Material for Broad Band White Light Emission.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Energetic and Structural Insights into Water Confined in Hydrophobic Nanopores.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Impact of Morphology and Composition of Graphene Aerosol-Gel Particles in Thin Films on Ultrafast Carrier Dynamics Studied via Transient Absorption Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Rapid Determination of SiO<sub>2</sub> Shell Thickness on Au Core Nanoparticles via Differential Centrifugal Sedimentation for SHINERS.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Effect of Exchange-Correlation Functionals on Schottky Barriers at Si/Metal Interfaces.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
See all related articles

Related Experiment Video

Updated: Nov 9, 2025

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
08:39

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Published on: October 16, 2017

12.9K

Complex Nanoparticle Diffusional Motion in Liquid-Cell Transmission Electron Microscopy.

Evangelos Bakalis1, Lucas R Parent2, Maria Vratsanos3

  • 1Dipartimento di Chimica "G. Ciamician", Universita di Bologna, V. F. Selmi 2, 40126 Bologna, Italy.

The Journal of Physical Chemistry. C, Nanomaterials and Interfaces
|April 12, 2021
PubMed
Summary
This summary is machine-generated.

Liquid-cell transmission electron microscopy (LCTEM) reveals nanoscale dynamics. Analyzing nanoparticle motion trajectories in LCTEM helps understand system conditions and avoid erroneous data interpretation.

More Related Videos

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
09:09

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Published on: March 5, 2021

4.6K
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.0K

Related Experiment Videos

Last Updated: Nov 9, 2025

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
08:39

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Published on: October 16, 2017

12.9K
Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
09:09

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Published on: March 5, 2021

4.6K
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.0K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Microscopy

Background:

  • Liquid-cell transmission electron microscopy (LCTEM) enables in situ observation of nanoscale solution-phase dynamics.
  • Artefactual effects from the liquid-cell environment can alter observed behavior compared to bulk conditions.
  • Accurate analysis of nanoparticle motion is crucial for understanding LCTEM experimental conditions.

Purpose of the Study:

  • To advance the anomalous diffusion object-motion analysis (ADOMA) method for LCTEM.
  • To extract detailed descriptions of liquid-cell system conditions during LCTEM experiments.
  • To improve the interpretation of nanoparticle diffusion and interaction data.

Main Methods:

  • Multistep analysis of object-motion trajectories from LCTEM data.
  • Independent and correlated analysis of x/y motion vectors.
  • Integration of object orientation/angle data into motion analysis.

Main Results:

  • The advanced ADOMA method provides a detailed description of LCTEM system conditions.
  • Analysis of motion trajectories reveals artefactual effects on nanoparticle behavior.
  • Independent and correlated vector analysis enhances understanding of confined diffusion.

Conclusions:

  • Careful analysis of motion data is essential to avoid misinterpreting LCTEM results.
  • The refined ADOMA method offers a robust approach to characterizing LCTEM environments.
  • This technique improves the reliability of nanoscale dynamic process observations in solution.