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Protein Dynamics in Living Cells

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Related Experiment Video

Updated: Nov 9, 2025

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

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Association of Nanodiamond Rotation Dynamics with Cell Activities by Translation-Rotation Tracking.

Xi Feng¹, Weng-Hang Leong¹, Kangwei Xia¹

¹Department of Physics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

|April 13, 2021

Summary

This summary is machine-generated.

Researchers developed a new method for tracking the full six-dimensional (6D) motion of single diamond particles. This technique reveals how particle rotation relates to live cell metabolic activities, advancing single particle tracking capabilities.

Keywords:

Cell membrane Nitrogen vacancy centers in nanodiamond Optically detected magnetic resonance Rotation tracking Single particle tracking

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Last Updated: Nov 9, 2025

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Area of Science:

Biophysics
Nanotechnology
Cell Biology

Background:

Understanding live system dynamics requires tracking particle movement and orientation.
Current single particle tracking methods often lack full six-dimensional (6D) motion analysis.
Nitrogen-vacancy (NV) centers in diamond offer unique sensing capabilities.

Purpose of the Study:

To develop and demonstrate a synchronized 3D translation and 3D rotation tracking technique for single particles.
To enable full 6D motion tracking of particles in biological systems.
To investigate the relationship between particle motion and cellular metabolic activity.

Main Methods:

Utilized nitrogen-vacancy (NV) center sensing in single diamond particles for synchronized motion tracking.
Performed 6D tracking of diamond particles attached to giant plasma membrane vesicles.
Applied the technique to monitor nanodiamonds on live cell membranes under varying physiological conditions.

Main Results:

Successfully achieved synchronized 3D translation and 3D rotation tracking of single diamond particles.
Quantitatively analyzed particle motion, isolating net rotation on vesicles.
Observed correlations between nanodiamond rotation and live cell metabolic activities.

Conclusions:

The developed technique provides a novel solution for 6D single particle tracking.
This method allows for critical correlated analysis of translation and rotation in biological dynamics.
Findings suggest particle rotation can serve as an indicator of cellular metabolic state.