Related Experiment Video
Updated: Nov 9, 2025
Creation and Maintenance of a Living Biobank - How We Do It
Published on: April 10, 2021
Study Protocol: Design and Implementation of the Pediatric Liver Transplantation Biobank
Zheng Xiang1,2, Ruijue Wang3, Yue Lu1,2
1Biobank Center of Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.
Insights
Establishing a pediatric liver transplantation (PLT) biobank provides critical samples for research. This standardized biobank supports early treatment of neonatal biliary atresia (BA) and other end-stage liver diseases, potentially reducing the need for liver transplants.
Area of Science:
- Pediatric Hepatology
- Biobanking
- Transplantation Science
Background:
- Early intervention for neonatal biliary atresia (BA) and end-stage liver diseases can mitigate the need for liver transplantation (LT).
- Standardized clinical pediatric liver transplantation (PLT) biobanks are essential for generating qualified sample resources for scientific research.
Purpose of the Study:
- To establish and describe a standardized clinical biobank for pediatric liver transplantation.
- To create a reliable platform for collecting and managing biospecimens and clinical data from pediatric LT patients.
Main Methods:
- Development of standardized procedures for biobank establishment, operations, and quality control.
- Systematic collection, management, and storage of liver tissue, blood, and stool samples from LT patients.
- Integration of biospecimen data with comprehensive clinical information.
Main Results:
- The biobank, operational since August 2018, has enrolled 49 pediatric subjects (ages 0-18).
- It currently houses approximately 3000 biospecimen aliquots.
- Biliary atresia (BA) is the most frequent diagnosis, accounting for 61.23% of LT cases.
Conclusions:
- The established PLT biobank serves as a valuable resource, linking detailed clinical and biological information.
- This resource is expected to significantly accelerate discovery research in pediatric liver transplantation.
- The biobank facilitates research into early treatment strategies for pediatric liver diseases.
Abstract:
Background: Early treatment of neonatal biliary atresia (BA) and other end-stage liver diseases can delay or prevent the necessity of liver transplantation (LT). The establishment of a standardized clinical pediatric liver transplantation (PLT) biobank is the prerequisite for scientific research, which helps to provide a qualified sample resource platform for research. Methods: Following standardized procedures to establish biobanks, the operational processes and quality control system were formulated. Liver tissue, blood, and stool samples undergoing LT were regularly collected, managed, and stored. Systematic management was conducted in collected specimens and corresponding clinical information. Results: Since implementation in August 2018, we have enrolled 49 unique subjects (0-18 years of age); the biobank contains nearly 3000 biospecimen aliquots. The most common LT diagnosis is BA (61.23%). Conclusion: The establishment of this biobank is a valuable resource that incorporates detailed clinical and biological information. It will help accelerate the pace of PLT discovery research. ClinicalTrials.gov ID: NCT04477967.
Related Concept Videos
01:28Kidney Transplant I: Introduction
01:26Kidney Transplant II: Surgical Procedure