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Related Experiment Video

Updated: Nov 9, 2025

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

Complement Detection in Mouse Kidneys by Immunofluorescence.

Jennifer Laskowski¹, Joshua M Thurman¹

¹Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 13, 2021

Summary

This summary is machine-generated.

This study details dual immunofluorescence staining of mouse kidney sections for complement proteins C3 and C4. It emphasizes careful tissue handling to preserve complement fragment integrity and enhance image quality.

Keywords:

Autofluorescence Complement Formalin-fixed and paraffin-embedded Immunofluorescence Microscopy

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Published on: June 8, 2022

Area of Science:

Immunology
Cell Biology
Histology

Background:

Immunofluorescence staining is a versatile technique for antigen detection and spatial analysis in tissues.
Complement proteins covalently fixed to tissues serve as durable markers of complement activation.
Detecting complement fragments via immunofluorescence requires specific handling protocols for unfixed tissues.

Purpose of the Study:

To describe a protocol for dual immunofluorescence staining of mouse kidney sections for complement proteins C3 and C4.
To highlight critical steps for preserving complement protein integrity during sample preparation.
To provide methods for improving signal-to-noise ratio for enhanced image quality.

Main Methods:

Dual immunofluorescence staining of frozen mouse kidney sections.
Specific protocols for handling unfixed tissues to preserve complement fragment integrity.
Optimization techniques to improve signal-to-noise ratio in immunofluorescence imaging.

Main Results:

Successful dual staining for complement proteins C3 and C4 in mouse kidney sections was achieved.
Key steps for preserving complement protein integrity were identified and emphasized.
Methods for improving signal-to-noise ratio were demonstrated to enhance image quality.

Conclusions:

A reliable protocol for dual immunofluorescence staining of complement proteins C3 and C4 in mouse kidneys is presented.
Proper tissue handling is crucial for accurate detection of complement fragments.
Optimized staining protocols improve the quality and interpretability of immunofluorescence data for complement research.