Related Experiment Videos
Chromatofocusing in the purification of staphylococcal enterotoxin D
Z Lei1, R F Reiser, M S Bergdoll
1Food Research Institute, University of Wisconsin, Madison 53706.
Journal of Clinical Microbiology
|June 1, 1988
Abstract:
A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.