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Related Experiment Video

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Electrical Impedance Spectroscopy for Microtissue Spheroid Analysis in Hanging-Drop Networks.

Yannick R F Schmid1, Sebastian C Bürgel1, Patrick M Misun1

  • 1Department of Biosystems Science and Engineering, Bio Engineering Laboratory, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.

ACS Sensors
|April 14, 2021
PubMed
Summary
This summary is machine-generated.

Electrical impedance spectroscopy (EIS) offers a label-free method for monitoring 3D tissue constructs. This study integrates EIS into a hanging-drop platform for real-time spheroid analysis, including growth and cardiac beating.

Keywords:
body on a chipcardiac spheroidimpedance sensormicroelectrodetumor growth

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Biosensing Technologies

Background:

  • Label-free and noninvasive monitoring of 3D tissue constructs is crucial for research.
  • Electrical impedance spectroscopy (EIS) is an emerging technique for biological analysis.
  • Hanging-drop platforms enable spheroid culture in perfused multitissue configurations.

Purpose of the Study:

  • To integrate an Electrical impedance spectroscopy (EIS) readout function into a hanging-drop network platform.
  • To demonstrate the capability of EIS for monitoring microtissue spheroids in situ.
  • To enable real-time analysis of microtissue growth and function.

Main Methods:

  • Integration of two pairs of microelectrodes into the hanging-drop support via a glass inlay.
  • Utilizing larger electrodes for drop size control and smaller electrodes for spheroid monitoring.
  • Implementing the system within a microfluidic structure for perfused multitissue cultures.

Main Results:

  • Successful integration of EIS readout into the hanging-drop network platform.
  • Demonstrated ability to monitor microtissue spheroid size, tracking growth (e.g., cancer microtissues).
  • Showcased capability to monitor cardiac microtissue beating in situ.

Conclusions:

  • The integration of EIS into hanging-drop networks is feasible.
  • This multifunctional platform allows for label-free, noninvasive monitoring of microtissues.
  • The system supports real-time analysis of microtissue development and function.