Related Experiment Video
Updated: Nov 9, 2025

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
Published on: May 2, 2016
Tissue distribution and cellular localization of gold nanocarriers with bound oligonucleotides
Nayab Fatima1, Ugur Akcan2, Mehmet Kaya2
1Department of Life, Health & Chemical Sciences, The Open University, Milton Keynes, UK.
Abstract:
Aim: The aim of the study was to determine how the addition of a DNA oligonucleotide cargo to 3-nm gold glyconanoparticles would affect tissue distribution. Methods: Gold glyconanoparticles with 1-6 covalently bound oligonucleotides (40 nt dsDNA) were injected into rats and allowed to circulate for 10 min. Organs were harvested and gold quantitated by inductively coupled plasma mass spectrometry. Cellular localization of the nanocarriers was determined by electron microscopy. Results & conclusion: Addition of DNA cargo to the nanocarriers prevented localization in the kidney but increased localization in liver hepatocytes and splenic macrophages. There was no significant change in heart, lung or brain. DNA increases the size and adds a strong negative charge to the nanoparticles, which radically affects tissue distribution.
Related Concept Videos
Tissue-Drug Binding: Localization of Drugs and its Significance
Drugs can bind to different tissue components, enhancing their distribution and localization. The factors influencing drug localization in tissues include the drug's lipophilicity, structural characteristics, tissue perfusion rate, and pH differences. These factors determine...
Drug Distribution: Tissue Binding
For...
Immunogold Electron Microscopy

