THE BALANCE OF IL-1β, IL-10 AND THE LEVEL OF IKBα EXPRESSION IN CHILDREN WITH CHRONIC CATARRHAL GINGIVITIS AND...

The aim: To define the level of pro- and anti-inflammatory IL-1β, IL-10 in the oral fluid of children with chronic gastroduodenitis, depending on the level of IkBα expression.

Materials and methods: Studiing the oral fluid and scraping of the gums of 50 children 6-12 years old was carried out to determine the level of IL-1β, IL-10 and IkBα. The children were divided into 3 groups. Group 1 - 10 children with healthy periodontium and without somatic diseases. 2nd group - 20 somatically healthy children with chronic catarrhal gingivitis. The third group (20 children) of children with gastroduodenitis and chronic catarrhal gingivitis. The determination of the mRNA expression of the IkBα geneand the level of IL-10 and IL-1β in the oral fluid was carried out by real-time PCR.

Results: We determined that the level of pro-inflammatory IL-1β in the oral fluid of primary school children had different levels in accordance with the state of dental and somatic health. It was the lowest in somatically healthy children without signs of gingivitis. We revealed that the level of IL-10 has anti-inflammatory properties, and an inverse relationship with the concentration of IL-1β in the oral fluid of examined children. Analysis of IkBα expression made it possible to reveal its suppression in children of the second and third groups compared with healthy children.

Conclusions: We found changes in the levels of IkBα in the gums of children with chronic catarrhal gingivitis; attenuated IkBα expression may lead to the deregulation of NF-κB pathways in the pathogenesis of periodontitis. Decreased IkBα expression may affect cytokine production and inflammatory response associated with chronic catarrhal gingivitis and with chronic gastroduodenitis.