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Related Experiment Video

Updated: Nov 9, 2025

Stress-induced Antibiotic Susceptibility Testing on a Chip
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Gradient-Based Microfluidic Platform for One Single Rapid Antimicrobial Susceptibility Testing.

Morteza Azizi1, Benyamin Davaji2, Ann V Nguyen1

  • 1Department of Food Science, College of Agricultural and Life Sciences, Cornell University, Stocking Hall, Ithaca, New York 14853, United States.

ACS Sensors
|April 14, 2021
PubMed
Summary
This summary is machine-generated.

A new microfluidic platform offers rapid antimicrobial susceptibility testing (AST) in just 3-4 hours. This innovative method enables faster diagnosis and treatment decisions for bacterial infections, addressing a critical need in healthcare.

Keywords:
antibiotic susceptibility testingfluorescence-based detectionmicrochambersmicrofluidicsresazurin

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Area of Science:

  • Microfluidics
  • Antimicrobial Resistance
  • Diagnostic Assays

Background:

  • Antimicrobial resistance (AMR) necessitates rapid antimicrobial susceptibility testing (AST) for effective treatment.
  • Conventional AST methods are time-consuming (24-72h), labor-intensive, and costly.
  • There is a need for faster, more efficient AST platforms.

Purpose of the Study:

  • To develop and validate a novel gradient-based microchamber microfluidic (GM2) platform for rapid AST.
  • To demonstrate the platform's ability to generate a wide range of antibiotic concentrations.
  • To profile bacteria relevant to human and animal health.

Main Methods:

  • A gradient-based microfluidic (GM2) platform was designed utilizing lateral diffusion of antibiotics.
  • Antibiotic concentration gradients were formed in microchambers by controlling fluid flow rates and concentrations.
  • The platform was tested with bacteria associated with human Crohn's disease and bovine mastitis.

Main Results:

  • The GM2 platform successfully generated a tunable gradient of antibiotic concentrations.
  • Complete AST assays were performed in approximately 3-4 hours.
  • Bacterial growth was tracked robustly, independent of antibiotic mechanism or species.

Conclusions:

  • The GM2 platform provides a rapid and efficient method for AST.
  • This technology has the potential to significantly improve in-clinic diagnostic capabilities.
  • The platform's versatility makes it suitable for diverse bacterial profiling applications.