Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.0K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.0K
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

523
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
523
Centrifugation01:05

Centrifugation

3.8K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
3.8K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

731
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
731
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

641
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
641
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

6.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fractionation of Extracellular Polymeric Substances by Aqueous Three-Phase Partitioning Systems.

Industrial & engineering chemistry research·2024
Same author

High-throughput Soxhlet extraction method applied for analysis of leaf lignocellulose and non-structural substances.

MethodsX·2024
Same author

Rhodotorula sp.-based biorefinery: a source of valuable biomolecules.

Applied microbiology and biotechnology·2022
Same author

A Paradoxical Role for Regulatory T Cells in the Tumor Microenvironment of Pancreatic Cancer.

Cancers·2022
Same author

Recovery Techniques Enabling Circular Chemistry from Wastewater.

Molecules (Basel, Switzerland)·2022
Same author

Complex coacervates as extraction media.

Green chemistry : an international journal and green chemistry resource : GC·2021
Same journal

Sustainable Refrigerants, Policy Drivers, and Emerging Technologies.

Annual review of chemical and biomolecular engineering·2026
Same journal

Introduction.

Annual review of chemical and biomolecular engineering·2026
Same journal

A Resonant Life.

Annual review of chemical and biomolecular engineering·2026
Same journal

Jamming and Yielding in Dense Suspensions.

Annual review of chemical and biomolecular engineering·2026
Same journal

Beyond Clean: Unraveling Phase Behavior and Rheology of Soaps.

Annual review of chemical and biomolecular engineering·2026
Same journal

The Nonequilibrium Self-Consistent Generalized Langevin Equation Theory of Glasses and Gels.

Annual review of chemical and biomolecular engineering·2026
See all related articles

Related Experiment Video

Updated: Nov 9, 2025

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.0K

Recent Developments in Solvent-Based Fluid Separations.

Boelo Schuur1, Thomas Brouwer1, Lisette M J Sprakel1

  • 1Sustainable Process Technology Group, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands; email: b.schuur@utwente.nl, T.Brouwer@utwente.nl, L.M.J.Sprakel@utwente.nl.

Annual Review of Chemical and Biomolecular Engineering
|April 14, 2021
PubMed
Summary
This summary is machine-generated.

This review highlights advancements in solvent-based separations, focusing on ionic liquids, bio-based solvents, and stimuli-responsive systems. Electrochemical methods show promise for carbon capture, biorefineries, and metals separations.

Keywords:
bio-based solventsdeep eutectic solventsfluid separationsgreen solventsionic liquidsstimuli-responsive solvents

More Related Videos

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
07:06

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Published on: May 14, 2020

5.3K
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.1K

Related Experiment Videos

Last Updated: Nov 9, 2025

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.0K
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
07:06

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Published on: May 14, 2020

5.3K
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.1K

Area of Science:

  • Chemical Engineering
  • Separation Science
  • Green Chemistry

Background:

  • Solvent-based fluid separations are critical across various industries.
  • Traditional molecular solvents face limitations in efficiency and environmental impact.

Purpose of the Study:

  • To review key developments in solvent-based fluid separations.
  • To discuss emerging solvent trends and their applications.
  • To highlight novel separation techniques.

Main Methods:

  • Review of recent literature on solvent-based separations.
  • Analysis of solvent trends: ionic liquids, deep eutectic solvents, bio-based solvents.
  • Discussion of stimuli-responsive systems (CO2-switchable, redox-responsive).

Main Results:

  • Ionic liquids and deep eutectic solvents are replacing traditional solvents.
  • Bio-based solvents are gaining traction in specific applications.
  • CO2-switchable and redox-responsive solvents show significant development.
  • Electrochemical separations offer new opportunities for carbon capture, biorefineries, and metals separations.

Conclusions:

  • The field of solvent-based separations is rapidly evolving with new solvent classes.
  • Stimuli-responsive solvents and electrochemical methods represent key future directions.
  • Sustainable and efficient separation technologies are crucial for environmental and industrial progress.