Related Experiment Video

Updated: Nov 9, 2025

Purification of Hsp104, a Protein Disaggregase
07:17

Purification of Hsp104, a Protein Disaggregase

Published on: September 30, 2011

17.6K

Release of Halophilic Extremozymes by Mechanical Cell Disruption

Mateja Primožič, Maja Čolnik, Željko Knez

    Acta Chimica Slovenica
    |April 15, 2021
    PubMed

    Insights

    Halophilic fungi, like Wallemia ichthyophaga, yield enzymes valuable for industry. Mechanical separation offers a novel method for obtaining these extremophilic enzymes for biotechnological applications.

    Area of Science:

    • Microbiology
    • Biotechnology
    • Enzymology

    Background:

    • Halophilic fungi, including Trimatostroma salinum, Wallemia ichthyophaga, Hortaea werneckii, and Phaeotheca triangularis, are extremophiles thriving in high salinity.
    • These fungi are a rich source of bioactive compounds, enzymes, and proteins with potential industrial applications in food and pharmaceuticals.

    Purpose of the Study:

    • To investigate the mechanical separation of enzymes from halophilic fungal cells.
    • To evaluate the potential of these enzymes for industrial applications, particularly in cascade reactions.

    Main Methods:

    • Mechanical separation techniques were employed to extract enzymes from halophilic fungal biomass.
    • The properties of enzymes from extremophiles were considered for their suitability under harsh conditions.

    Main Results:

    • A method for separating enzymes as a cocktail from halophilic fungi was successfully developed.
    • The study highlights the biotechnological significance of these enzyme cocktails for industrial use.

    Conclusions:

    • Mechanical separation provides an effective means to obtain enzyme cocktails from halophilic fungi.
    • Enzymes from extremophiles, due to their enhanced stability, are promising for industrial processes operating under challenging conditions.

    More Related Videos

    Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation10:34

    Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

    Published on: February 1, 2018

    9.4K
    Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill14:00

    Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

    Published on: January 29, 2021

    8.5K

    Related Concept Videos

    Tissue Homogenization and Cell Lysis01:32

    Tissue Homogenization and Cell Lysis

    Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
    Mechanical methods of tissue homogenization
    These methods rely on applying external physical force to disrupt...
    9.0K
    Lysosomal Hydrolases01:22

    Lysosomal Hydrolases

    Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
    4.2K
    Diversity of Archaea IV01:29

    Diversity of Archaea IV

    Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
    219
    DNA Isolation01:24

    DNA Isolation

    DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
    43.1K
    Transformation01:26

    Transformation

    Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
    368
    Diversity of Archaea III01:27

    Diversity of Archaea III

    Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
    180