Effects of different antifreeze chemicals on late spring frost in pistachio

Frost injury is one of the major limiting factors to horticultural crops production and distribution. Despite numerous efforts and researches concerning freezing injury reduction, it still accounts for more than 50% of the fruit losses in the horticulture sector. In the present investigation, we aimed to investigate the effects of different antifreeze compounds (Tiofer®, Cropaid®, Bio-Bloom®, amino acid (mixture), salicylic acid, and water (control)) on pistachio trees behavior under low-temperature regimes (2 °C, 0 °C, -2 °C, -4 °C, -6 °C, and spring natural temperature). The applied chemicals improved the osmolyte content during the cold stress. Tiofer® and Cropaid® could increase the proline content better than other compounds. Salicylic acid and Cropaid® application increased the guaiacol peroxidase (GPX) content better than other compounds. For ascorbate peroxidase (APX), Tiofer® and Bio-Bloom®, and for catalase (CAT), Tiofer®, Cropaid®, and salicylic acid performed better. Applying chemicals also improved the photosynthetic pigments under cold stress. Among all treatments, Tiofer® and Bio-Bloom® improved the chlorophyll a (Chla), while chlorophyll b (Chlb) better improved by Tiofer® and Cropaid®; moreover, carotenoids had better increase in Cropaid®, amino acid, and salicylic acid treatments. All applied chemicals except Tiofer® had a good effect on the anthocyanin content increase under cold stress. In conclusion, based on the findings presented here, applying antifreeze compounds, such as Tiofer®, Cropaid® Bio-Bloom®, salicylic acid, and amino acid, could effectively ameliorate the adverse effects of cold stress. Osmolytes and antioxidant (GPX, APX, CAT) contents, photosynthetic pigments (chlorophyll a and b and carotenoid), and anthocyanins were improved. Among all applied antifreezes, Tiofer® and Cropaid® were the most effective ones.