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Updated: Nov 9, 2025
RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Published on: June 4, 2016
The complete genome sequence of Listeria monocytogenes strain S2542 and expression of selected genes under
Ilhan Cem Duru1, Florentina Ionela Bucur2, Margarita Andreevskaya3,4
1Institute of Biotechnology, University of Helsinki, Helsinki, Finland. ilhan.duru@helsinki.fi.
Objectives:
The study aims to generate the whole genome sequence of L. monocytogenes strain S2542 and to compare it to the genomes of strains RO15 and ScottA. In addition, we aimed to compare gene expression profiles of L. monocytogenes strains S2542, ScottA and RO15 after high-pressure processing (HPP) using ddPCR.
Results:
The whole genome sequence of L. monocytogenes S2542 indicates that this strain belongs to serotype 4b, in contrast to the previously reported serotype 1/2a. Strain S2542 appears to be more susceptible to the treatment at 400 MPa compared to RO15 and ScottA strains. In contrast to RO15 and ScottA strains, viable cell counts of strain S2542 were below the limit of detection after HPP (400 MPa/8 min) when stored at 8 °C for 24 and 48 h. The transcriptional response of all three strains to HPP was not significantly different.
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