Related Experiment Video
Updated: Nov 9, 2025
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
Published on: June 1, 2019
9.3K
Dynamic Intracellular Metabolic Cell Signaling Profiles During Ag-Dependent B-Cell Differentiation
Paula Díez1,2, Martín Pérez-Andrés1, Martin Bøgsted3
1Department of Medicine and Cytometry General Service-Nucleus, CIBERONC, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca, Spain.
Frontiers in Immunology
|April 16, 2021
Summary
This study provides the first proteomic analysis of human B-cell maturation. Key differences in protein expression, particularly in metabolic regulation, were identified across B-cell subpopulations during antigen-dependent differentiation.
Area of Science:
- Immunology
- Proteomics
- Cell Biology
Background:
- Human B-cell differentiation is well-studied at genomic and transcriptomic levels.
- Detailed proteomic analysis of antigen-dependent B-cell maturation populations is lacking.
Purpose of the Study:
- To conduct the first quantitative proteomic profiling of human B-cell subpopulations during antigen-dependent maturation.
- To identify protein expression changes associated with B-cell differentiation stages.
Main Methods:
- Label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed.
- Five purified human B-cell subpopulations (naive, centroblasts, centrocytes, memory, plasma B-cells) from tonsils were analyzed.
- Proteomic data were deposited in ProteomeXchange (identifier PXD006191).
Main Results:
- Differences among B-cell subpopulations involve specific protein expression and maturation-associated changes.
- Protein expression alterations are linked to metabolic regulation.
- Significant proteomic overlap exists across the five B-cell subsets.
Conclusions:
- B-cell maturation involves stage-specific proteins and altered protein expression levels, particularly in metabolic regulation.
- Regulation of molecular stoichiometry in metabolic pathways is crucial for B-cell differentiation.
- Signaling cascades including antigen recognition, presentation, and cell survival are vital for B-cell maturation stages.
Related Concept Videos
01:24B Cell Activation and Differentiation
14.8K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
14.8K
01:22Diversity in Cell Signaling Responses
7.2K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...
Graded and Abrupt Responses
Some signaling systems generate...
7.2K
01:22T Cell Activation and Clonal Selection
13.7K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
13.7K
01:23Cells of the Adaptive Immune Response
7.3K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
7.3K
01:26Signal Transduction: Overview
10.2K
Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
Typically, signal transduction involves three...
10.2K
01:15Differentiation of Common Myeloid Progenitor Cells
3.6K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.6K