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Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
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Persister cells as a possible cause of antibiotic therapy failure in Helicobacter pylori

Parvin Bahmaninejad1, Sobhan Ghafourian1, Mina Mahmoudi1

  • 1Department of Microbiology, Faculty of Medicine Ilam University of Medical Sciences Ilam Iran.

JGH Open : an Open Access Journal of Gastroenterology and Hepatology
|April 16, 2021
PubMed
Abstract

Insights

Persister cells in Helicobacter pylori infections may cause treatment failure and recurrence. Further research is needed to understand their role in H. pylori infections.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Antibiotic treatment failure and recurrence of Helicobacter pylori infections are significant clinical challenges.
  • Understanding the underlying causes is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the potential role of persister cells in H. pylori treatment failure and recurrence.
  • To identify the prevalence of H. pylori and antibiotic susceptibility in Ilam, Iran.

Main Methods:

  • Biopsy specimens from 100 patients with H. pylori symptoms were analyzed.
  • H. pylori identification, antibiotic susceptibility assays, and persister cell assays were performed.
  • Patients with persister cells were monitored post-treatment for infection recurrence.

Main Results:

  • H. pylori was detected in 50% of patients.
  • Persister cells were found in 18% of susceptible isolates.
  • These persister cells showed sensitivity to clarithromycin at concentrations 5-fold higher than the MIC.

Conclusions:

  • Persister cells are implicated as a potential cause of recurrent H. pylori infections and antibiotic treatment failure.
  • Further investigation is warranted to fully elucidate the mechanisms and implications of H. pylori persister cells.
Keywords:
Helicobacter pyloridrug tolerancereinfection

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