Transcriptomic analysis of diapause-associated genes in Exorista civilis Rondani (Diptera:Tachinidae)
Bo Zhang1, Hai-Bin Han1, Lin-Bo Xu1
1Research Center for Quality, Safety and Risk Assessment of Grass and Livestock Products, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.
Archives of Insect Biochemistry and Physiology
|April 16, 2021
Summary
This study reveals key genes and metabolic pathways involved in the diapause of Exorista civilis (Diptera: Tachinidae). Transcriptome analysis provides a foundation for understanding the molecular basis of insect diapause.
Area of Science:
- Entomology
- Molecular Biology
- Genomics
Background:
- Exorista civilis (Diptera: Tachinidae) is a globally significant parasitic insect.
- Limited research exists on the molecular regulation of diapause in E. civilis.
Purpose of the Study:
- To identify diapause-associated genes and metabolic pathways in E. civilis.
- To establish a theoretical basis for understanding diapause molecular mechanisms at the transcriptome level.
Main Methods:
- Transcriptome sequencing using the Illumina HiSeq. 2000 platform.
- Bioinformatics analysis of non-diapause and diapause pupae.
- Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
Main Results:
- Assembly of 58,050 unigenes, with 4,355 upregulated and 3,158 downregulated differentially expressed unigenes.
- Identification of diapause-associated genes involved in cold resistance, amino acid metabolism, and energy metabolism pathways.
- Validation of five upregulated genes' expression patterns using quantitative real-time polymerase chain reaction (qRT-PCR) and RNA-Seq.
Conclusions:
- The study elucidates crucial molecular players and pathways regulating diapause in E. civilis.
- Provides a foundation for future research into the genetic and metabolic underpinnings of insect diapause.
