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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.

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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for...

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Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

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Preference testing for UV light spectrum and intensity in laying hens.

Md Sohel Rana¹, Andrew M Cohen-Barnhouse², Caroline Lee²

¹School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia; Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Armidale, New South Wales 2350, Australia; Department of Livestock Services, Ministry of Fisheries and Livestock, Dhaka 1215, Bangladesh.

Poultry Science

|April 19, 2021

Summary

Ultraviolet B (UVB) light may encourage free-range hens to use outdoor areas. However, intense sunlight may require hens to seek shelter, suggesting a need for protective measures in free-range environments.

Keywords:

UV light behavior free-range laying hen light preference sunlight

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Area of Science:

Animal Behavior
Avian Science
Photobiology

Background:

Free-range hens' outdoor usage is influenced by sunlight intensity and UV radiation.
The specific light aspect (brightness vs. UV) deterring hens from outdoor access is unclear.

Purpose of the Study:

To determine hen preference for different light wavelengths and intensities.
To investigate the impact of light treatments on outdoor range usage behavior.

Main Methods:

Controlled indoor choice study with cage-reared ISA Brown laying hens (n=84).
Hens were exposed to standard LED white light (control) versus visible spectrum plus infrared (VIS), UVA, or UVA/UVB light treatments at varying intensities.
Preference was measured by time spent in each compartment, analyzed using Generalized Linear Mixed Models (GLMM).

Main Results:

Hens preferred low-intensity UVA/UVB, low-intensity VIS, medium-intensity UVA/UVB, medium-intensity UVA, and high-intensity VIS light over control.
Feeding behavior decreased under all UVA light intensities.
Foraging, ground pecking, and preening increased under low-intensity UVA/UVB light.

Conclusions:

UVA/UVB light, mimicking sunlight, may positively influence hen range use.
During peak sun intensity, hens might require protective measures like shelter.
Further research in free-range settings is necessary to confirm these findings.