Electrochemical Impedance Spectroscopy of Zinc Oxide Nanoparticles After Deposition on Screen Printed Electrode

A small aliquot (10-14 μL) of ZnO nanoparticles dispersed in deionized water was deposited by evaporation to produce a dry residue on the working area of a screen-printed electrode. An electrochemical test solution containing K₃Fe(CN)₆ and KCl was added to the electrode surface for analysis by electrochemical impendence spectroscopy (EIS). Using this deposition analysis technique, a new relationship between the charge transfer resistance (R_ct) and the amount of ZnO nanoparticles has been explored. Based on the trend of increasing R_ct value with an increase of ZnO nanoparticles, a quantitative analysis method can be established to determine the mass of nanoparticles (0.01-1.00 μg) deposited from an unknown dispersion. To study the matrix effect, addition of Nafion solution to the aqueous dispersion resulted in a change of the linear range to 0.3-0.5 μg nanoparticles. Addition of methanol (10% by volume) to the aqueous dispersion changes the analysis range to 0.2-0.6 μg nanoparticles, while additional methanol (50% by volume) changes the analysis range to 0.06-1.00 μg nanoparticles. The analytical sensitivity, as indicated by the slope of each standard calibration curve, ranked as: aqueous dispersion > Nafion/aqueous dispersion > 10% methanol/aqueous dispersion > 50% methanol/aqueous dispersion. Altogether these results verify that deionized water is the best dispersion medium for EIS analysis of ZnO nanoparticles.