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CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Nov 8, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Amplification-free RNA detection with CRISPR-Cas13.

Hajime Shinoda¹, Yuya Taguchi¹, Ryoya Nakagawa²

¹Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan.

Communications Biology

|April 20, 2021

Summary

CRISPR-based amplification-free digital RNA detection (SATORI) offers rapid, sensitive RNA detection in under 5 minutes. This novel platform enhances diagnostic speed and accuracy for molecular targets like SARS-CoV-2.

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Area of Science:

Molecular Biology
Biotechnology
Diagnostics

Background:

CRISPR-based nucleic acid detection is promising for molecular diagnostics but often requires lengthy pre-amplification steps.
Pre-amplification can introduce errors and increase assay time, limiting real-time diagnostic applications.

Purpose of the Study:

To develop a rapid, amplification-free CRISPR-based platform for digital RNA detection.
To enhance sensitivity and specificity for molecular diagnostics using CRISPR-Cas13 technology.

Main Methods:

Combined CRISPR-Cas13 RNA detection with microchamber-array technology to create the SATORI platform.
Utilized single-stranded RNA targets and multiple guide RNAs for enhanced detection.

Main Results:

Achieved maximal sensitivity of approximately 10 femtomolar (fM) for single-stranded RNA targets in under 5 minutes.
Demonstrated high specificity and enabled detection of SARS-CoV-2 N-gene RNA at approximately 5 fM levels through simultaneous use of multiple guide RNAs.

Conclusions:

The SATORI platform provides accurate and rapid amplification-free digital RNA detection.
SATORI has the potential to significantly advance molecular diagnostics, offering a powerful tool for timely disease detection.