Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.7K
Reporter Genes02:11

Reporter Genes

12.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metal-Phenolic Coatings Enable Universal Design of Spherical Nucleic Acids.

Angewandte Chemie (International ed. in English)·2026
Same author

High-χ Block Copolymer Nanoreactors for the Confined Synthesis of Size-Controlled Nanoclusters.

ACS nano·2026
Same author

Programmable Stepwise Heteroepitaxial Growth of Colloidal Crystals With Different Phases.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Simplex-based model for nanoparticle grain identification in four-dimensional scanning transmission electron microscopy data.

Journal of microscopy·2026
Same author

High-entropy 1D halide perovskite piezoelectrics found by megalibrary synthesis and rapid nonlinear optical screening.

Science advances·2026
Same author

Using Well-Defined DNA Nanostructures To Study the Influence of DNA Clustering and Presentation on SNA Cellular Uptake.

Nano letters·2026
Same journal

Efficient Syngas Photoproduction Enabled by Electronic Engineering of Co-Immobilized Imine COFs.

Angewandte Chemie (International ed. in English)·2026
Same journal

Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.

Angewandte Chemie (International ed. in English)·2026
Same journal

Interaction Hierarchy and Polymorphic Structure-Property Dynamics in Luminescent Molecular Crystals.

Angewandte Chemie (International ed. in English)·2026
Same journal

The Role of Zn-Hf Site Proximity and Oxygen Vacancies for Methanol Formation Over ZnHfO<sub>x</sub> Catalysts Under CO<sub>2</sub> Hydrogenation Conditions.

Angewandte Chemie (International ed. in English)·2026
Same journal

Breaking the Linear Scaling Relationship: Bioinspired Electronic Coupling in S-Bridged Fe-Fe Dual Sites for Efficient Oxygen Reduction.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Related Experiment Video

Updated: Nov 8, 2025

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

16.2K

Programming Fluorogenic DNA Probes for Rapid Detection of Steroids.

Sasha B Ebrahimi1, Devleena Samanta2, Benjamin E Partridge2

  • 1Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL, 60208, USA.

Angewandte Chemie (International Ed. in English)
|April 20, 2021
PubMed
Summary
This summary is machine-generated.

A new aptamer detection strategy, forced intercalation (FIT), uses computational design to create fluorescent sensors. This method accurately detects steroids like dehydroepiandrosterone sulfate (DHEAS) in clinical samples rapidly and with minimal volume.

Keywords:
DNAaptamersdetectionforced intercalationsteroids

More Related Videos

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

10.2K
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
06:26

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

3.3K

Related Experiment Videos

Last Updated: Nov 8, 2025

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

16.2K
ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

10.2K
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
06:26

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

3.3K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Aptamers are versatile recognition agents for detecting molecules in complex environments.
  • Current aptamer detection methods often rely on significant structural changes upon target binding.
  • There is a need for sensitive and efficient aptamer-based detection strategies.

Purpose of the Study:

  • To develop a novel aptamer-based detection strategy, forced intercalation (FIT), for enhanced molecular recognition.
  • To create fluorescent aptamer sensors using computational modeling and screening.
  • To demonstrate the utility of FIT-aptamers for detecting steroids in clinical samples.

Main Methods:

  • Designed and screened a library of green-fluorescent forced intercalation aptamers (FIT-aptamers) guided by computational modeling.
  • Utilized subtle aptamer structural changes upon target binding for fluorescent signal transduction.
  • Quantified steroid detection using a standard plate reader with low sample volumes.

Main Results:

  • Identified FIT-aptamers capable of sensing dehydroepiandrosterone sulfate (DHEAS) at concentrations as low as 1.3 μM.
  • FIT-aptamers exhibited comparable binding affinity to unmodified aptamers.
  • Demonstrated rapid (<30 min) and sensitive detection of DHEAS in clinical serum samples.

Conclusions:

  • The forced intercalation (FIT) strategy significantly expands the scope of aptamer-based detection by transducing subtle structural changes into fluorescent signals.
  • FIT-aptamers offer a sensitive, rapid, and instrumentally simple method for steroid detection in biological samples.
  • This approach holds promise for the development of advanced diagnostic tools using aptamer technology.