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Oxidative Cleavage of Alkenes: Ozonolysis

Oxidative Cleavage of Alkenes: Ozonolysis

In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

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Related Experiment Video

Updated: Nov 8, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

[In-situ Sludge Reduction Technology Based on Ozonation].

Bing Xue¹, Bin-Han Liu¹, Ting-Ting Wei¹

¹College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.

Huan Jing Ke Xue= Huanjing Kexue

|April 22, 2021

Summary

Ozone treatment (O3/MLVSS) at 75 mg·g-1 with a 10-day sludge age reduced excess sludge by 12% in wastewater treatment. Microbial communities shifted, impacting nitrification/denitrification but maintaining effluent quality.

Keywords:

dewaterability microbial community ozone parameter sedimentation performance sludge

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Published on: September 11, 2016

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

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Published on: December 25, 2015

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Last Updated: Nov 8, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency

Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency

Published on: September 11, 2016

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Area of Science:

Environmental Engineering
Water Treatment Technologies
Microbial Ecology

Context:

Investigating in-situ sludge reduction using ozone in wastewater treatment systems.
Evaluating the impact of ozone dosage and sludge age on process parameters and sludge properties.

Purpose:

To determine optimal ozone dosage and sludge age for effective in-situ sludge reduction.
To analyze the effects of ozone treatment on sludge yield, microbial community structure, and effluent quality.

Summary:

An ozone dosage of 75 mg·g-1 (O3/MLVSS) and a sludge age of 10 days were identified as optimal conditions, achieving a 12% reduction in excess sludge.
Ozone treatment altered microbial populations, increasing Bacteroidetes and decreasing Proteobacteria, while macromolecular organic substances in EPS increased, affecting sludge dewaterability.
Despite shifts in microbial communities and some impact on sedimentation, the system maintained stable operation and met effluent discharge standards.

Impact:

Provides optimized parameters for in-situ sludge reduction, potentially reducing operational costs and environmental impact.
Offers insights into the microbial and physicochemical changes in sludge due to ozone treatment.
Demonstrates the feasibility of ozone for sludge reduction while maintaining wastewater treatment efficiency and compliance.