Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optical photothermal infrared (O-PTIR) micro-spectroscopic characterisation of breast cancer cell lines: a comparative molecular profile of MCF7, MDA-MB-231, and SKBR3 cells.

The Analyst·2026

Same author

Beyond Color: Hybrid Vibrational-Electronic Broadband Coherent Anti-Stokes Raman Scattering for Molecularly Informed Digital Pathology.

Analytical chemistry·2026

Same author

SORS and SESORS: deep Raman spectroscopy in biomedical analysis and disease diagnosis.

Chemical Society reviews·2026

Same author

Toward real-time protein profiling in biofluids using discriminative Raman spectral markers.

The Analyst·2026

Same author

An Artificial Intelligence-Based Screening Tool for Detection of Cerebral Venous Thrombosis on Non-Contrast Brain CT.

Cerebrovascular diseases (Basel, Switzerland)·2026

Same author

Micro-SORS and machine learning for the non-invasive reference-free study of subsurface pigment degradation.

The Analyst·2026

Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026

Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026

Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026

Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026

Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026

Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 8, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Spatially Offset Raman Spectroscopy-How Deep?

Sara Mosca¹, Priyanka Dey², Marzieh Salimi²

¹Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, UK Research and Innovation, Harwell Campus, Didcot OX11 0QX, United Kingdom.

Analytical Chemistry

|April 22, 2021

Summary

This summary is machine-generated.

Spatially offset Raman spectroscopy (SORS) noninvasively probes subsurface sample composition. This study reveals the reduced scattering coefficient dictates SORS probing depth, enabling precise depth selection for various applications.

More Related Videos

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Related Experiment Videos

Last Updated: Nov 8, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Area of Science:

Optics and Spectroscopy
Materials Science
Non-invasive Analysis

Background:

Spatially offset Raman spectroscopy (SORS) is a powerful non-invasive technique for analyzing the subsurface composition of turbid materials.
A critical challenge in SORS is determining the probing depth for a given spatial offset or selecting an appropriate offset for a desired depth.

Purpose of the Study:

To establish a method for predicting and controlling the probing depth in SORS measurements.
To provide a framework for optimizing spatial offset selection in SORS experiments.

Main Methods:

Utilized Monte Carlo simulations to model light propagation in turbid media, assuming negligible absorption.
Identified the reduced scattering coefficient as the primary parameter influencing the probed zone's extent in point-like SORS.
Validated the simulation results with experimental data from stratified, both absorbing and non-absorbing, samples.

Main Results:

Demonstrated that the reduced scattering coefficient of the medium is the key factor determining the SORS probing depth.
Showcased that this coefficient can be obtained from literature or estimated directly from SORS measurements.
Provided simulation data correlating spatial offset with probed depth, applicable to various SORS configurations.

Conclusions:

A straightforward methodology is presented to determine the probing depth in SORS based on the reduced scattering coefficient.
This approach enhances the interpretation and application of SORS data across diverse fields.
The findings are directly relevant for optimizing SORS in biomedical, pharmaceutical, security, forensic, and cultural heritage applications.