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Updated: Nov 8, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration.

N Contessi Negrini¹, A Angelova Volponi², C A Higgins¹

¹Department of Bioengineering, Imperial College London, London, UK.

Materials Today. Bio

|April 23, 2021

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Developmental tissue engineering (TE) mimics embryonic development to regenerate tissues. This approach shows promise for creating functional ectodermal appendages like teeth and hair follicles using advanced 3D scaffolds.

Area of Science:

Regenerative Medicine
Developmental Biology
Biomaterials Science

Background:

Classical tissue engineering (TE) struggles with clinical translation due to inadequate construct features.
Ectodermal appendages develop via complex epithelial-mesenchymal interactions (secondary induction).

Purpose of the Study:

To review the emergence and achievements of developmental TE.
To focus on 3D scaffolds for regenerating ectodermal appendages (teeth, hair follicles, glands).

Main Methods:

Mimicking in vivo morphogenetic processes using developmental TE strategies.
Utilizing 3D scaffolds, specific cell sources, and optimized cell culture configurations.
Reviewing biomaterial selection and cell culture configurations for developmental TE.

Keywords:

Cell coculture Developmental Epithelial-mesenchymal interaction Gland regeneration Hair follicle regeneration Tooth regeneration tissue engineering

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Last Updated: Nov 8, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Published on: March 24, 2011

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies

Published on: November 5, 2016

Main Results:

Developmental TE successfully replicates in vivo morphogenetic cues for ectodermal appendage regeneration.
Successful in vitro and in vivo applications reported for teeth, hair follicles, and glands.
Accurate selection of biomaterials and cell culture configurations is crucial for developmental TE.

Conclusions:

Developmental TE offers a novel paradigm for creating tissues with correct biomorphology and biofunctionality.
This approach holds significant potential for regenerating ectodermal appendages.
Further research in biomaterials and cell culture is essential for advancing developmental TE.