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Updated: Nov 8, 2025

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
Published on: May 21, 2020
Genome and transcriptome of a pathogenic yeast, Candida nivariensis
Yunfan Fan1, Andrew N Gale2, Anna Bailey1
1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Abstract:
We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.
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