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Updated: Nov 8, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

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MiR-124-3p Suppresses Prostatic Carcinoma by Targeting PTGS2 Through the AKT/NF-κB Pathway

Zhilei Zhang1

  • 1Department of Urologic Surgery, Shengli Hospital of Dongying, No.107 Beier Road, Dongying District, Dongying, 257000, Shandong, China. zzlgood2007@sina.com.

Molecular Biotechnology
|April 24, 2021
PubMed

Insights

MicroRNA-124-3p (miR-124-3p) exhibits anti-tumor effects in prostate cancer by targeting PTGS2 and inhibiting the AKT/NF-κB pathway. This suggests miR-124-3p is a potential therapeutic target for prostate carcinoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNA-124-3p (miR-124-3p) has demonstrated tumor-regulatory functions in various cancers.
  • The specific roles of miR-124-3p in prostate carcinoma remain incompletely understood.

Purpose of the Study:

  • To investigate the regulatory functions of miR-124-3p in prostate carcinoma.
  • To explore the potential of miR-124-3p as a therapeutic target for prostate cancer.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry, and western blot were used to assess expression levels.
  • Dual-luciferase reporter assay confirmed the interaction between miR-124-3p and PTGS2.
  • Cell viability, proliferation, migration, invasion, and apoptosis were evaluated using MTT, colony formation, and flow cytometry assays.

Main Results:

  • Prostate carcinoma tissues and cells showed decreased miR-124-3p and increased PTGS2 expression.
  • PTGS2 was identified as a direct target of miR-124-3p.
  • miR-124-3p suppressed prostate carcinoma cell viability, proliferation, migration, and invasion while enhancing apoptosis by inhibiting the AKT/NF-κB pathway via PTGS2.

Conclusions:

  • miR-124-3p exerts anti-tumor effects in prostate carcinoma by targeting PTGS2 and inactivating the AKT/NF-κB pathway.
  • miR-124-3p presents potential as an emerging therapeutic target for prostate carcinoma treatment.
Keywords:
AKT/NF-κBMiR-124-3pPTGS2Prostatic carcinoma

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