Fluorescent sensing for some nitric oxide donors in dosage forms and biological matrices

New fluorescent sensing of some nitric oxide donors, nitroglycerin and isosorbide dinitrate was developed in our laboratories. Two fluorescent reagents, 2-(2-hydroxyethylamino)-4,6-dimethylpyridine-3-carbonitrile, 3a and 2-(3-chloro-phenylamino)-4,6-dimethylpyridine-3-carbonitrile, 3b were synthesized in our laboratories and a comparative study was performed between them from the point of fluorescence intensity. The fluorophore, 3a, was selected for the analytical study as it exhibit higher quantum yield value. The interaction between the selected drugs and the fluorophore was noticed to be quenching. The mechanism of quenching was studied and it was supposed to be collisional quenching through photo induced electron transfer process. The proposed sensing method was applied successfully for the analysis of nitroglycerin and isosorbide dinitrate in dosage forms within concentration range of (0.05-0.5 µg/mL) with percentage recoveries of 99.9 ± 0.5 and 99.9 ± 0.7 respectively. The studied drugs, nitroglycerin and isosorbide dinitrate, were also probed in spiked biological matrices such as plasma samples with percentage recoveries of 99.1 ± 1.97 and 100.7 ± 1.96 and urine samples with percentage recoveries of 100.4 ± 1.8 and 100.3 ± 1.7 respectively. In vivo analysis of both drugs in real plasma was also investigated. The sensing method exhibit well intra-day and inter day precision with %RSD < 2%.