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Related Experiment Video

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Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging
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mmSIM: an open toolbox for accessible structured illumination microscopy.

Craig T Russell1, Michael Shaw2,3

  • 1EBI, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge CB10 1SD, UK.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|April 26, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces open-source software for super-resolution structured illumination microscopy (SIM), simplifying the control of custom-built SIM systems. The software aims to make advanced bioimaging techniques more accessible for researchers.

Keywords:
FOSScontrol softwarefluorescence microscopymicro-managerstructured illumination microscopy

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Area of Science:

  • Microscopy
  • Biotechnology
  • Optical Engineering

Background:

  • Super-resolution structured illumination microscopy (SIM) is a powerful bioimaging technique.
  • Commercial SIM systems are expensive and inflexible, leading to the development of many custom-built systems.
  • Existing open-source tools lack integrated software for controlling SIM hardware and synchronizing components.

Purpose of the Study:

  • To develop and present a suite of open-source software tools for controlling custom-built SIM systems.
  • To simplify the process of developing and operating SIM hardware.
  • To support the wider adoption of super-resolution structured illumination microscopy.

Main Methods:

  • Developed a new software suite based on the Micro-Manager package.
  • Utilized the software to control two custom-built, high-speed, spatial light modulator-based SIM systems.
  • Evaluated system performance by imaging various fluorescent samples.

Main Results:

  • The new software effectively controls custom-built SIM systems.
  • Demonstrated the capability of the developed SIM systems through imaging experiments.
  • The software simplifies the synchronization of critical hardware components for SIM imaging.

Conclusions:

  • The developed open-source software facilitates the creation and operation of custom SIM systems.
  • This work aims to lower the barrier to entry for super-resolution microscopy.
  • Simplifying SIM hardware development can promote broader use of this advanced imaging technique.