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Updated: Nov 8, 2025
Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
Published on: November 17, 2017
Differential data on the responsiveness of multiple cell types to cell death induced by non-thermal atmospheric
Ko Eto1, Chiaki Ishinada1, Takuya Suemoto1
1Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Japan.
Abstract:
A discovery that cells die of a novel and distinctive process, along with some characteristic events, such as cellular shrinkage and Programmed cell death 4 disappearance, has been done by using non-thermal atmospheric pressure plasma-activated solutions [1]. Data on the responsiveness of multiple cell types to the induction of cellular shrinkage and cell death and the loss of Programmed cell death 4 by exposure to the non-thermal atmospheric pressure plasma-activated solutions were collected. Human neuroblastoma SH-SY5Y cells, murine myoblast C2C12 cells, and murine embryonic fibroblasts were cultured for various periods in each of the non-thermal atmospheric pressure plasma-activated solutions and then examined by light field microscopic observation for their effects on cell morphology, by Trypan blue dye exclusion assay for those on cell death, and by Western blotting for those on Programmed cell death 4 disappearance. The data clarified some differences in the responsiveness to the induction of cellular shrinkage, cell death, and Pdcd4 disappearance by all the non-thermal atmospheric pressure plasma-activated solutions among the cells.
Insights
Non-thermal atmospheric pressure plasma-activated solutions induce a novel cell death process, characterized by shrinkage and Programmed cell death 4 (Pdcd4) loss. Different cell types exhibit varying responses to this plasma-induced cell death.
Area of Science:
- Biophysics
- Cell Biology
- Plasma Medicine
Background:
- Non-thermal atmospheric pressure plasma (NTAPP) is emerging as a tool in biomedical applications.
- Cell death pathways are crucial in biological processes and disease.
- Programmed cell death 4 (Pdcd4) is a key regulator involved in apoptosis.
Purpose of the Study:
- To investigate a novel cell death process induced by NTAPP-activated solutions.
- To characterize the morphological and molecular events associated with this cell death.
- To assess the differential responsiveness of various cell types to NTAPP-induced cell death.
Main Methods:
- Cell culture of human neuroblastoma SH-SY5Y, murine myoblast C2C12, and murine embryonic fibroblasts.
- Exposure of cells to NTAPP-activated solutions.
- Microscopic observation for cell morphology changes.
- Trypan blue dye exclusion assay for cell viability assessment.
- Western blotting to detect Programmed cell death 4 (Pdcd4) levels.
Main Results:
- NTAPP-activated solutions induced a distinctive cell death process.
- Characteristic events included cellular shrinkage and Programmed cell death 4 (Pdcd4) disappearance.
- Variations in responsiveness to NTAPP-induced cell death were observed across different cell types.
Conclusions:
- NTAPP-activated solutions can induce a novel form of cell death.
- Cellular shrinkage and Pdcd4 loss are key indicators of this process.
- Cell type-specific responses highlight the complexity of plasma-cell interactions.
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