Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Swab Testing to Optimize Pneumonia Treatment With Empiric Vancomycin: A Randomized Controlled Trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Neuropeptide Y receptor modulation of dopamine transmission in the nucleus accumbens core.

Neuropeptides·2026
Same author

Circular RNA <i>circHomer1</i> mediates hippocampal functions via ribonucleoprotein granule transport and dendritic targeting of synaptic RNAs.

Science advances·2026
Same author

Cannabidiol Reduces D1 and D2 Medium Spiny Neuron Excitability in the Nucleus Accumbens Core.

bioRxiv : the preprint server for biology·2025
Same author

Leveraging TRPV1 for intracellular delivery of membrane impermeant compounds in the brain.

Scientific reports·2025
Same author

Identification of photoperiod as a regulator of dopamine-mediated behavior in female mice.

Neurobiology of sleep and circadian rhythms·2025

Related Experiment Video

Updated: Nov 8, 2025

Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation
09:39

Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation

Published on: June 7, 2016

10.8K

Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices.

Kevin M Manz1,2,3, Justin K Siemann2,4, Douglas G McMahon2,4,5

  • 1Medical Scientist Training Program, Vanderbilt University, Nashville, TN 37232, USA.

STAR Protocols
|April 26, 2021
PubMed
Summary
This summary is machine-generated.

This guide details electrophysiology techniques like patch-clamp and multi-electrode arrays for studying neuron function. It provides a protocol for preparing rodent brain slices for novice and expert electrophysiologists.

Keywords:
Cell BiologyNeuroscienceSignal Transduction

More Related Videos

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
10:24

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Published on: January 10, 2015

17.6K
Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity
11:56

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity

Published on: November 11, 2017

16.0K

Related Experiment Videos

Last Updated: Nov 8, 2025

Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation
09:39

Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation

Published on: June 7, 2016

10.8K
Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
10:24

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Published on: January 10, 2015

17.6K
Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity
11:56

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity

Published on: November 11, 2017

16.0K

Area of Science:

  • Neuroscience
  • Electrophysiology

Background:

  • Patch-clamp and multi-electrode array electrophysiology are essential for measuring dynamic neuronal functional properties.
  • These techniques allow for detailed investigation of cellular and synaptic parameters governing neurotransmission.

Purpose of the Study:

  • To provide a comprehensive guide for preparing acute rodent brain slices.
  • To demonstrate example experiments and analyses using patch-clamp and multi-electrode array electrophysiology.
  • To cater to both novice and expert electrophysiologists.

Main Methods:

  • Utilizing whole-cell and cell-attached patch-clamp recordings in voltage-clamp and current-clamp configurations.
  • Employing multi-electrode array electrophysiology for recording spike activity from multiple neurons.
  • Preparing acute rodent brain slices for electrophysiological analysis.

Main Results:

  • Demonstration of cell-type-specific synaptic and cellular parameters influencing neurotransmission.
  • Capability for larger sample sizes and long-term recordings using multi-electrode array electrophysiology.
  • Successful preparation of acute rodent brain slices for functional analysis.

Conclusions:

  • The protocol enables detailed study of neuronal function using established electrophysiological methods.
  • This guide serves as a valuable resource for researchers in the field of neuroscience.
  • Accurate preparation of brain slices is crucial for reliable electrophysiological recordings.