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Updated: Nov 8, 2025

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Published on: June 12, 2020

Modeling human peri-implantation placental development and function†.

J Zhou^1,2, R C West³, E L Ehlers¹

¹Mizzou Institute for Women's Health Research, Department of Obstetrics, Gynecology and Women's Health, University of Missouri School of Medicine, Columbia, MO USA.

Biology of Reproduction

|April 26, 2021

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Studying early human pregnancy is challenging due to lack of clinical detection. New models, including extended blastocyst culture and stem-cell derived trophoblast culture, offer insights into peri-implantation placental development.

Area of Science:

Reproductive Biology
Developmental Biology
Stem Cell Biology

Background:

Human implantation and early placental development are poorly understood due to limited access to peri-implantation stage pregnancies.
Existing animal and in vitro models for studying human placentation have significant limitations.

Purpose of the Study:

To present and discuss two contemporary models for studying human peri-implantation placental development: extended blastocyst culture and stem-cell derived trophoblast culture.
To highlight current research utilizing these models and their future potential for investigating the critical "black box" stage of early human pregnancy.

Main Methods:

Extended blastocyst culture: Culturing human blastocysts for extended periods to observe early post-implantation events.
Stem-cell derived trophoblast culture: Differentiating human stem cells into trophoblast cells to model placental development.

Keywords:

extended blastocyst culture human implantation placenta pluripotent stem cells trophoblast stem cells (TSC)

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Main Results:

These models provide novel avenues for investigating the earliest stages of human placental formation.
They overcome limitations of previous models, offering better accessibility to the peri-implantation period.

Conclusions:

Extended blastocyst culture and stem-cell derived trophoblast culture represent significant advancements in studying early human placental development.
These models hold promise for unraveling the complexities of the "black box" period in human pregnancy, advancing reproductive science.